Anti-Transferrin Antibody (3I436), Serum Loading Control is a Mouse antibody targeting Transferrin. Anti-Transferrin Antibody (3I436), Serum Loading Control can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

3I436

Human,Rat,Mouse,Cow

1. Blank control (black line): HepG2.
Primary Antibody (green line): Mosue Anti-Transferrin (Serum Loading Control) antibody (TMAB-13729)
Dilution: 1 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-AF488
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (TMAB-13729) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human skin cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Transferrin) Monoclonal Antibody, Unconjugated (TMAB-13729) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
4. 25 μg total protein per lane of various lysates (see on figure) probed with Transferrin monoclonal antibody, unconjugated (TMAB-13729) at 1:5000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:5000-10000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test

Monoclonal

Full length native Transferrin protein purified from human plasma

Human

TF

Serotransferrin

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.