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Anti-TOMM20 Antibody (6C702)
😃Good
Catalog No. TMAC-04077
Alias Translocase of outer mitochondrial membrane 20 homolog(yeast), Translocase of outer mitochondrial membrane 20 homolog type II, TOM20, TOM 20, Outer mitochondrial membrane receptor Tom20, MOM19, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, MGC117367, MAS20, KIAA0016
Anti-TOMM20 Antibody (6C702) is a Rabbit antibody targeting TOMM20. Anti-TOMM20 Antibody (6C702) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-TOMM20 Antibody (6C702)
Anti-TOMM20 Antibody (6C702)
😃Good
Catalog No. TMAC-04077Alias Translocase of outer mitochondrial membrane 20 homolog(yeast), Translocase of outer mitochondrial membrane 20 homolog type II, TOM20, TOM 20, Outer mitochondrial membrane receptor Tom20, MOM19, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, MGC117367, MAS20, KIAA0016
Anti-TOMM20 Antibody (6C702) is a Rabbit antibody targeting TOMM20. Anti-TOMM20 Antibody (6C702) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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| Description | Anti-TOMM20 Antibody (6C702) is a Rabbit antibody targeting TOMM20. Anti-TOMM20 Antibody (6C702) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | Translocase of outer mitochondrial membrane 20 homolog(yeast), Translocase of outer mitochondrial membrane 20 homolog type II, TOM20, TOM 20, Outer mitochondrial membrane receptor Tom20, MOM19, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, MGC117367, MAS20, KIAA0016 |
| Ig Type | IgG |
| Clone | 6C702 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of TOMM20 on different lysates using anti-TOMM20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: MCF-7, Lane 3: F9, Lane 4: PC12. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. The nuclear counter stain is DAPI (blue). 6. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TOMM20 antibody. Counter stained with hematoxylin. 7. ICC staining TOMM20 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining TOMM20 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining TOMM20 in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with TOMM20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The mitochondrial preprotein translocases of the outer membrane (Tom) is a multisubunit protein complex that facilitates the import of nucleus-encoded precursor proteins across the mitochondrial outer membrane. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. The import receptors include Tom20 and Tom22, which form a heteromeric receptor complex that initiates the insertion of newly synthesized proteins into the outer membrane and then directs the precursor protein into the GIP. In yeast, Tom22 is the essential component of the import receptor complex as it functions as both a receptor for the preproteins and serves as a docking point for both Tom20 and the GIP. Tom22 directly associates with Tom40, the major component of the GIP, and thereby forms a stable interaction between the two core complexes to facilitate the fluid movement of preproteins into the mitochondria. The insertion of Tom40 into the Tom machinery requires the initial binding of Tom40 to Tom20 and leads to the efficient incorporation of Tom40 precursors into preexisting Tom complexes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 16 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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