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Anti-TNF RII Antibody (5O99)
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Catalog No. TMAC-04065
Alias Tumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, p75
Anti-TNF RII Antibody (5O99) is a Rabbit antibody targeting TNF RII. Anti-TNF RII Antibody (5O99) can be used in WB,ICC/IF,IHC,FCM,IP.
Anti-TNF RII Antibody (5O99)
Anti-TNF RII Antibody (5O99)
😃Good
Catalog No. TMAC-04065Alias Tumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, p75
Anti-TNF RII Antibody (5O99) is a Rabbit antibody targeting TNF RII. Anti-TNF RII Antibody (5O99) can be used in WB,ICC/IF,IHC,FCM,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-TNF RII Antibody (5O99) is a Rabbit antibody targeting TNF RII. Anti-TNF RII Antibody (5O99) can be used in WB,ICC/IF,IHC,FCM,IP. |
| Synonyms | Tumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, p75 |
| Ig Type | IgG |
| Clone | 5O99 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of TNF Receptor II on different cells lysates using anti-TNF Receptor II antibody at 1/500 dilution. Positive control: Line 1: MCF-7, Line 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue anti-TNF Receptor II antibody. Counter stained with hematoxylin. 5. ICC staining TNF Receptor II in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining TNF Receptor II in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining TNF Receptor II in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of HL-60 cells with TNF Receptor II antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Tumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types. The majority of TNF functions are primarily mediated through TNF-R1, while signaling through TNF-R2 occurs less extensively and is confined to cells of the immune system. Both of these proteins belong to the growing TNF and nerve growth factor (NGF) receptor superfamily, which includes FAS, CD30, CD27 and CD40. The members of this superfamily are type I membrane proteins that share sequence homology confined to the extracellular region. TNF-R1 shares a motif termed the "death domain" with FAS and three structurally unrelated signaling proteins, TRADD, FADD and RIP (1,3-8). This death domain is required for transduction of the apoptotic signal. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 73 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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