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Anti-TNF RII Antibody (5O99) (Synonyms: Tumor necrosis factor receptor type II, Tumor necrosis factor receptor ...)

Catalog No. TMAC-04065 Copy Product Info
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Anti-TNF RII Antibody (5O99) is a Rabbit antibody targeting TNF RII. Anti-TNF RII Antibody (5O99) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-TNF RII Antibody (5O99)

Copy Product Info
🥰Excellent
Catalog No. TMAC-04065
Synonyms Tumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, p75

Anti-TNF RII Antibody (5O99) is a Rabbit antibody targeting TNF RII. Anti-TNF RII Antibody (5O99) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-TNF RII Antibody
(5O99)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2967-10 days7-10 days
100 μL$4977-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-TNF RII Antibody (5O99) is a Rabbit antibody targeting TNF RII. Anti-TNF RII Antibody (5O99) can be used in FCM,ICC/IF,IHC,IP,WB.
SynonymsTumor necrosis factor receptor type II, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, p75
Ig Type
IgG
Clone
5O99
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of TNF Receptor II on different cells lysates using anti-TNF Receptor II antibody at 1/500 dilution. Positive control: Line 1: MCF-7, Line 2: Jurkat.
2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue anti-TNF Receptor II antibody. Counter stained with hematoxylin.
5. ICC staining TNF Receptor II in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining TNF Receptor II in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining TNF Receptor II in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. Flow cytometric analysis of HL-60 cells with TNF Receptor II antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:10-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundTumor necrosis factor (TNF) is a pleiotropic cytokine whose function is mediated through two distinct cell surface receptors. These receptors, designated TNF-R1 and TNF-R2, are expressed on most cell types. The majority of TNF functions are primarily mediated through TNF-R1, while signaling through TNF-R2 occurs less extensively and is confined to cells of the immune system. Both of these proteins belong to the growing TNF and nerve growth factor (NGF) receptor superfamily, which includes FAS, CD30, CD27 and CD40. The members of this superfamily are type I membrane proteins that share sequence homology confined to the extracellular region. TNF-R1 shares a motif termed the "death domain" with FAS and three structurally unrelated signaling proteins, TRADD, FADD and RIP (1,3-8). This death domain is required for transduction of the apoptotic signal.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 73 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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