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Anti-TGF-beta 3 Polyclonal Antibody
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Catalog No. TMAB-13499 Copy Product Info
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Anti-TGF-beta 3 Polyclonal Antibody is a Rabbit antibody targeting TGF-beta 3. Anti-TGF-beta 3 Polyclonal Antibody can be used in WB,FCM.
Anti-TGF-beta 3 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 µL | $220 | 7-10 days | 7-10 days | |
| 100 µL | $374 | 7-10 days | 7-10 days | |
| 200 µL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
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| Description | Anti-TGF-beta 3 Polyclonal Antibody is a Rabbit antibody targeting TGF-beta 3. Anti-TGF-beta 3 Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat) |
| Verified Activity | 1. Sample: Ovary (Mouse) Lysate at 40 μg Primary: Anti-TGF-beta 3 (TMAB-13499) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30'47 kD Observed band size: 47 kD 2. Blank control (black line): sH-SY5Y. Primary Antibody (green line): Rabbit Anti-TGF-beta 3 antibody (TMAB-13499) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-AF488 Dilution: 0.5 μg/Test. Negative control (white blue line): PBS Isotype control (orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| Concentration | 1mg/ml |
| Research Background | Transforming growth factor beta 3(TGF β3) is one of three closely related mammalian members of the large TGF β superfamily, TGF-β1, β2, and β3, signal through the same receptor and elicit similar biological responses. The mammalian TGF-β3 controls a vast array of biological processes including immune regulation, cell proliferation, epithelial-mesenchymal transition, and the bone formation. It is generally recognized to facilitate chondrogenic differentiation of precursor cells. It may also have a dose-dependent inhibitory effect on osteogenesis. Recombinant Human TGF-β3 is a 25.0 kDa protein composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. |
| Immunogen | KLH conjugated synthetic peptide: human TGF-beta 3 |
| Antigen Species | Human |
| Gene Name | TGFB3 |
| Gene ID | |
| Protein Name | Transforming growth factor beta-3 proprotein |
| Uniprot ID | |
| Function | Involved in embryogenesis and cell differentiation. |
| Molecular Weight | Theoretical: 30/47 kDa. Actual: 47 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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