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Anti-TGF beta 1/2/3 Polyclonal Antibody
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Catalog No. TMAB-01828
Anti-TGF beta 1/2/3 Polyclonal Antibody is a Rabbit antibody targeting TGF beta 1/2/3. Anti-TGF beta 1/2/3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Anti-TGF beta 1/2/3 Polyclonal Antibody
Anti-TGF beta 1/2/3 Polyclonal Antibody
😃Good
Catalog No. TMAB-01828
Anti-TGF beta 1/2/3 Polyclonal Antibody is a Rabbit antibody targeting TGF beta 1/2/3. Anti-TGF beta 1/2/3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Sheep,GuineaPig) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Sheep,GuineaPig) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-TGF Beta 1+2+3 Polyclonal Antibody, Unconjugated (TMAB-01828) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Spleen (Rabbit) Lysate at 40 μg Primary: Anti-TGF Beta 1+2+3 (TMAB-01828) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution Predicted band size: 12.8/44 kDa Observed band size: 44 kDa 3. Sample: Stomach (mouse) Lysate at 40 μg Primary: Anti-TGF Beta 1+2+3 (TMAB-01828) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kDa Observed band size: 44 kDa 4. Blank control: K562. Primary Antibody (green line): Rabbit Anti-TGF Beta 1+2+3 antibody (TMAB-01828) Dilution: 2 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. |
| Application | WB,IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. |
| Immunogen | KLH conjugated synthetic peptide: human TGF Beta 1 |
| Antigen Species | Human |
| Gene ID | 7040 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,TGF,Angiogenic growth factors,TGF,Co-factors,Hypoxia,tgf beta 1,TGF,Secreted |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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