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Anti-TCF7L2 Antibody (8D555)
😃Good
Catalog No. TMAC-03995
Alias Transcription factor 7-like 2, Transcription factor 7 like 2 T cell specific HMG box, Transcription factor 7 like 2, TF7L2_HUMAN, TCF7L2 protein, TCF7L2, TCF-4, TCF4, TCF 4, T-cell-specific transcription factor 4, T-cell factor 4, T cell specific transcription factor 4, T cell specific HMG box, T cell factor 4, hTCF-4, hTCF 4, HMG box transcription factor 4
Anti-TCF7L2 Antibody (8D555) is a Rabbit antibody targeting TCF7L2. Anti-TCF7L2 Antibody (8D555) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-TCF7L2 Antibody (8D555)
Anti-TCF7L2 Antibody (8D555)
😃Good
Catalog No. TMAC-03995Alias Transcription factor 7-like 2, Transcription factor 7 like 2 T cell specific HMG box, Transcription factor 7 like 2, TF7L2_HUMAN, TCF7L2 protein, TCF7L2, TCF-4, TCF4, TCF 4, T-cell-specific transcription factor 4, T-cell factor 4, T cell specific transcription factor 4, T cell specific HMG box, T cell factor 4, hTCF-4, hTCF 4, HMG box transcription factor 4
Anti-TCF7L2 Antibody (8D555) is a Rabbit antibody targeting TCF7L2. Anti-TCF7L2 Antibody (8D555) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $298 | 7-10 days | 7-10 days | |
| 100 μL | $497 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Chemical Properties
| Description | Anti-TCF7L2 Antibody (8D555) is a Rabbit antibody targeting TCF7L2. Anti-TCF7L2 Antibody (8D555) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | Transcription factor 7-like 2, Transcription factor 7 like 2 T cell specific HMG box, Transcription factor 7 like 2, TF7L2_HUMAN, TCF7L2 protein, TCF7L2, TCF-4, TCF4, TCF 4, T-cell-specific transcription factor 4, T-cell factor 4, T cell specific transcription factor 4, T cell specific HMG box, T cell factor 4, hTCF-4, hTCF 4, HMG box transcription factor 4 |
| Ig Type | IgG |
| Clone | 8D555 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of TCF7L2 on Jurkat cells lysates using anti-TCF7L2 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin. 4. ICC staining TCF7L2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining TCF7L2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining TCF7L2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Jurkat cells with TCF7L2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.        |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to β-catenin in a cooperative manner. This TCF-4/β-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes. c-Jun also interacts with TCF-4 and β-catenin, and the phosphorylation-dependent interaction between c-Jun and TCF4 regulates intestinal tumorigenesis by integrating JNK and APC/β-catenin. TCF-4 is also implicated in bipolar affective disorder. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 68 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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