Anti-TBL1XR1 Antibody (6Q913) is a Rabbit antibody targeting TBL1XR1. Anti-TBL1XR1 Antibody (6Q913) can be used in FCM, ICC/IF, WB.

IgG

6Q913

Human (predicted:Mouse,Rat)

1. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (TBL1XR1) monoclonal Antibody, unconjugated (TMAB-13396) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-TBL1XR1 antibody (TMAB-13396,1:50); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 25 μg total protein per lane of various lysates (see on figure) probed with TBL1XR1 monoclonal antibody, unconjugated (TMAB-13396) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

FCM=1:50-100; ICC/IF=1:50-200; WB=1:500-2000

Monoclonal

A synthesized peptide: human TBL1XR1

Human

TBL1XR1

F-box-like/WD repeat-containing protein TBL1XR1

F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of the N-Cor corepressor complex that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of N-Cor complex, thereby allowing cofactor exchange, and transcription activation.