Anti-SNAI1 Antibody (6G916) is a Rabbit antibody targeting SNAI1. Anti-SNAI1 Antibody (6G916) can be used in FCM, WB.

IgG

6G916

Human

1. The A549 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-SNAI1 antibody (TMAB-12994, 1: 100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 25 ug total protein per lane of various lysates (see on figure) probed with SNAI1 monoclonal antibody, unconjugated (TMAB-12994) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

FCM=1 μg/Test; WB=1:500-2000

Monoclonal

Recombinant Protein: human SNAI1 protein

SNAI1

Zinc finger protein SNAI1

Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm. Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.