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Anti-SKP1 Antibody (9W207)

😃Good
Catalog No. TMAC-03768
Alias TCEB1L, S-phase kinase-associated protein 1, SKP1A, p19A, OCP-II, OCP2, EMC19

Anti-SKP1 Antibody (9W207) is a Rabbit antibody targeting SKP1. Anti-SKP1 Antibody (9W207) can be used in WB,ICC/IF,FCM.

Anti-SKP1 Antibody (9W207)

Anti-SKP1 Antibody (9W207)

😃Good
Catalog No. TMAC-03768Alias TCEB1L, S-phase kinase-associated protein 1, SKP1A, p19A, OCP-II, OCP2, EMC19
Anti-SKP1 Antibody (9W207) is a Rabbit antibody targeting SKP1. Anti-SKP1 Antibody (9W207) can be used in WB,ICC/IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2977-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-SKP1 Antibody (9W207) is a Rabbit antibody targeting SKP1. Anti-SKP1 Antibody (9W207) can be used in WB,ICC/IF,FCM.
SynonymsTCEB1L, S-phase kinase-associated protein 1, SKP1A, p19A, OCP-II, OCP2, EMC19
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Skp1 on PC-12 cell using anti-Skp1 antibody at 1/1,000 dilution.
2. ICC staining Skp1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
3. ICC staining Skp1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
4. Flow cytometric analysis of Hela cells with Skp1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Application
Recommended Dose
WB: 1:500; ICC/IF: 1:50-100; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundEssential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 19 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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