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Anti-SHP-1 Antibody (7A74)
😃Good
Catalog No. TMAC-03746
Alias SH-PTP1, SHP-1, PTPTY-42, Ptp1C, protein tyrosine phosphatase, non-receptor type 6, me, Hcph, hcp, 70Z-SHP
Anti-SHP-1 Antibody (7A74) is a Rabbit antibody targeting SHP-1. Anti-SHP-1 Antibody (7A74) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-SHP-1 Antibody (7A74)
Anti-SHP-1 Antibody (7A74)
😃Good
Catalog No. TMAC-03746Alias SH-PTP1, SHP-1, PTPTY-42, Ptp1C, protein tyrosine phosphatase, non-receptor type 6, me, Hcph, hcp, 70Z-SHP
Anti-SHP-1 Antibody (7A74) is a Rabbit antibody targeting SHP-1. Anti-SHP-1 Antibody (7A74) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $496 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-SHP-1 Antibody (7A74) is a Rabbit antibody targeting SHP-1. Anti-SHP-1 Antibody (7A74) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | SH-PTP1, SHP-1, PTPTY-42, Ptp1C, protein tyrosine phosphatase, non-receptor type 6, me, Hcph, hcp, 70Z-SHP |
| Ig Type | IgG |
| Clone | 7A74 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of SHP1 on Raji cell lysates using anti-SHP1 antibody at 1/500 dilution. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-SHP1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SHP1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SHP1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SHP1 antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-SHP1 antibody. Counter stained with hematoxylin. 8. ICC staining SHP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining SHP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of Hela cells with SHP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.           |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 68 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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