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Anti-Sequestosome-1 Antibody (4X151) (Synonyms: Ubiquitin-binding protein p62, SQSTM, Sequestosome-1, phospho...)
Catalog No. TMAC-03710 Copy Product Info
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Anti-Sequestosome-1 Antibody (4X151) is a Mouse antibody targeting Sequestosome-1. Anti-Sequestosome-1 Antibody (4X151) can be used in FCM,ICC，IHC.
Anti-Sequestosome-1 Antibody (4X151)
Copy Product Info🥰Excellent
Catalog No. TMAC-03710
Synonyms Ubiquitin-binding protein p62, SQSTM, Sequestosome-1, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, p60), OSIL, ORCA, EBI3-associated protein of 60 kDa(EBIAP
Anti-Sequestosome-1 Antibody (4X151) is a Mouse antibody targeting Sequestosome-1. Anti-Sequestosome-1 Antibody (4X151) can be used in FCM,ICC，IHC.
Anti-Sequestosome-1 Antibody
(4X151)
(4X151)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $296 | 7-10 days | 7-10 days | |
| 100 μL | $427 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Sequestosome-1 Antibody (4X151) is a Mouse antibody targeting Sequestosome-1. Anti-Sequestosome-1 Antibody (4X151) can be used in FCM,ICC，IHC. |
| Synonyms | Ubiquitin-binding protein p62, SQSTM, Sequestosome-1, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, p60), OSIL, ORCA, EBI3-associated protein of 60 kDa(EBIAP |
| Clone | 4X151 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution. Positive control: Lane1: Hela, Lane2: HepG2. 2. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin. 5. ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela?cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.       |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:200; ICC: 1:200; FCM: 1 μg per 1 x 106 cells |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Peptide affinity purified. |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. |
| Conjucates | Unconjugated |
| Immunogen | KLH conjugated synthetic peptide: within human SQSTM1 40-100 aa |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 62 kDa. |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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