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Anti-SEMA4D Antibody (7E489)
😃Good
Catalog No. TMAY-02073
Alias semaphorin 4D, SEMAJ, M-sema-G, coll-4, COLL4, CD100, C9orf164
Anti-SEMA4D Antibody (7E489) is a Mouse antibody targeting SEMA4D. Anti-SEMA4D Antibody (7E489) can be used in FCM.
Anti-SEMA4D Antibody (7E489)
Anti-SEMA4D Antibody (7E489)
😃Good
Catalog No. TMAY-02073Alias semaphorin 4D, SEMAJ, M-sema-G, coll-4, COLL4, CD100, C9orf164
Anti-SEMA4D Antibody (7E489) is a Mouse antibody targeting SEMA4D. Anti-SEMA4D Antibody (7E489) can be used in FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: FCM Reactivity: Human |
| Alias | semaphorin 4D, SEMAJ, M-sema-G, coll-4, COLL4, CD100, C9orf164 |
| Ig Type | Mouse IgG1 |
| Clone | 7E489 |
| Reactivity | Human |
| Verified Activity | Flow cytometric analysis of Human SEMA4D(CD100) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. |
| Application | FCM |
| Recommended Dose | FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Semaphorin 4D / SEMA4D / CD100 (rh Semaphorin 4D / SEMA4D / CD100; TMPY-01906; AAH54500.1; Met1-Arg734). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Semaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties. |
| Conjucates | Unconjugated |
| Others Formats | PE |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Human Semaphorin 4D / SEMA4D / CD100 Protein (TMPY-01906) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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