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Anti-SEMA4D Antibody (7E489)

😃Good
Catalog No. TMAY-02073
Alias semaphorin 4D, SEMAJ, M-sema-G, coll-4, COLL4, CD100, C9orf164

Anti-SEMA4D Antibody (7E489) is a Mouse antibody targeting SEMA4D. Anti-SEMA4D Antibody (7E489) can be used in FCM.

Anti-SEMA4D Antibody (7E489)

Anti-SEMA4D Antibody (7E489)

😃Good
Catalog No. TMAY-02073Alias semaphorin 4D, SEMAJ, M-sema-G, coll-4, COLL4, CD100, C9orf164
Anti-SEMA4D Antibody (7E489) is a Mouse antibody targeting SEMA4D. Anti-SEMA4D Antibody (7E489) can be used in FCM.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-SEMA4D Antibody (7E489) is a Mouse antibody targeting SEMA4D. Anti-SEMA4D Antibody (7E489) can be used in FCM.
Aliassemaphorin 4D, SEMAJ, M-sema-G, coll-4, COLL4, CD100, C9orf164
Ig Type
Monoclonal Mouse IgG1
Clone
7E489
Reactivity
Human
Verified Activity
Flow cytometric analysis of Human SEMA4D(CD100) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
Application
FCM
Recommended Dose
FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Semaphorin 4D / SEMA4D / CD100 (rh Semaphorin 4D / SEMA4D / CD100; TMPY-01906; AAH54500.1; Met1-Arg734). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundSemaphorin 4D (SEMA4D or CD100) is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
PE
Antibody Types Available
2
Antigen Details
Immunogen
Recombinant Human Semaphorin 4D / SEMA4D / CD100 Protein (TMPY-01906)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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