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Anti-SCF Antibody (9H58)
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Catalog No. TMAC-03691
Alias Stem cell factor, Mast cell growth factor, Kit ligand, c-Kit ligand
Anti-SCF Antibody (9H58) is a Rabbit antibody targeting SCF. Anti-SCF Antibody (9H58) can be used in FCM,ICC/IF,IHC,WB.
Anti-SCF Antibody (9H58)
Anti-SCF Antibody (9H58)
😃Good
Catalog No. TMAC-03691Alias Stem cell factor, Mast cell growth factor, Kit ligand, c-Kit ligand
Anti-SCF Antibody (9H58) is a Rabbit antibody targeting SCF. Anti-SCF Antibody (9H58) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-SCF Antibody (9H58) is a Rabbit antibody targeting SCF. Anti-SCF Antibody (9H58) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | Stem cell factor, Mast cell growth factor, Kit ligand, c-Kit ligand |
| Ig Type | IgG |
| Clone | 9H58 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of SCF on Raji cells lysates using anti-SCF antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SCF antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SCF antibody. Counter stained with hematoxylin. 4. ICC staining SCF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining SCF in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining SCF in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of MCF-7 cells with SCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:100-500; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Stem cell factor (SCF) is the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit. SCF, also designated KL, MGF and SLF, is a pleiotropic cytokine that has two alternatively spliced forms, 248 and 220 amino acids in length in human and mouse systems, respectively. Both the larger form and the smaller form are cleaved to produce soluble forms. The smaller splice variant lacks the proteolytic cleavage site, between amino acids 149 and 177 of the larger SCF form. The larger form of SCF is expressed in fibroblasts, brain and thymus, while the smaller form is found in spleen, testis, placenta and cerebellum. The SCF ligand is essential for the development of germ cells, hematopoietic progenitor cells and melanocyte precursors. SCF can stimulate the proliferation of mature mast cells as well as the maturation and proliferation of immature mast cells. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 37 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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