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Anti-RRM1 Antibody (3D202)
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Catalog No. TMAY-02718
Alias RR1, RIR1, ribonucleotide reductase M1, R1
Anti-RRM1 Antibody (3D202) is a Mouse antibody targeting RRM1. Anti-RRM1 Antibody (3D202) can be used in FCM,ICC/IF.
Anti-RRM1 Antibody (3D202)
Anti-RRM1 Antibody (3D202)
😃Good
Catalog No. TMAY-02718Alias RR1, RIR1, ribonucleotide reductase M1, R1
Anti-RRM1 Antibody (3D202) is a Mouse antibody targeting RRM1. Anti-RRM1 Antibody (3D202) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-RRM1 Antibody (3D202) is a Mouse antibody targeting RRM1. Anti-RRM1 Antibody (3D202) can be used in FCM,ICC/IF. |
| Alias | RR1, RIR1, ribonucleotide reductase M1, R1 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 3D202 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human RRM1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human RRM1 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. 2. Flow cytometric analysis of Human RRM1 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human RRM1, then a PE-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RRM1 (rh RRM1; TMPY-03287; P23921; Met1-Ser792). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | RRM1 is a subunit of ribonucleoside-diphosphate reductase which is constituted by two subunits. Ribonucleoside-diphosphate reductase is an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. RRM1 is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. RRM1 may play a role in malignancies and disease that involve this region. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human RRM1 protein (TMPY-03287) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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