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Anti-RNF20 Antibody (1Z606) is a Rabbit antibody targeting RNF20. Anti-RNF20 Antibody (1Z606) can be used in WB,IP,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-RNF20 Antibody (1Z606) is a Rabbit antibody targeting RNF20. Anti-RNF20 Antibody (1Z606) can be used in WB,IP,FCM. |
Synonyms | RNF 20, RING-type E3 ubiquitin transferase BRE1A, RING finger protein 20, hBRE1, EC 2.3.2.27, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A |
Ig Type | IgG |
Reactivity | Human,Mouse |
Verified Activity | 1. Western blot analysis of RNF20 on different lysates using anti-RNF20 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: MCF-7, Lane 3: Jurkat. 2. Flow cytometric analysis of K562 cells with RNF20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000; ICC: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). RNF20 (ring finger protein 20), also known as BRE1, BRE1A or hBRE1, is a 975 amino acid nuclear protein that belongs to the BRE1 family. As a component of the RNF20/40 complex, RNF20 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such as Histone H2B. RNF20 is required for transcriptional activation of Hox genes and is most likely recruited by p53 to the MDM2 promoter, thereby acting as a transcriptional co-activator. RNF20 contains one zinc finger domain and exists as a homodimer. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 114 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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