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Anti-RBP4 Antibody (2R164)
😃Good
Catalog No. TMAY-03155
Alias retinol binding protein 4, plasma
Anti-RBP4 Antibody (2R164) is a Rabbit antibody targeting RBP4. Anti-RBP4 Antibody (2R164) can be used in FCM,ICC/IF.
Anti-RBP4 Antibody (2R164)
Anti-RBP4 Antibody (2R164)
😃Good
Catalog No. TMAY-03155Alias retinol binding protein 4, plasma
Anti-RBP4 Antibody (2R164) is a Rabbit antibody targeting RBP4. Anti-RBP4 Antibody (2R164) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-RBP4 Antibody (2R164) is a Rabbit antibody targeting RBP4. Anti-RBP4 Antibody (2R164) can be used in FCM,ICC/IF. |
| Alias | retinol binding protein 4, plasma |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 2R164 |
| Reactivity | Mouse |
| Verified Activity | 1. Flow cytometric analysis of Mouse RBP4 expression on BABL/c splenocytes. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Mouse RBP4, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 2. Immunofluorescence staining of mouse RBP4 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse RBP4 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse RBP4 (rM RBP4; TMPY-01567; NP_035385.1; Met1-Leu201). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). |
| Conjucates | Unconjugated |
| Others Formats | FITC |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Mouse RBP4 protein (TMPY-01567) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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