Anti-Pygopus 2 Antibody (3Q571) is a Rabbit antibody targeting Pygopus 2. Anti-Pygopus 2 Antibody (3Q571) can be used in FCM, ICC/IF.

IgG

3Q571

Human (predicted:Mouse)

1. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.). Primary Antibody (green): Rabbit Anti-Pygopus 2 antibody (TMAB-11959, 1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488: 1 μg/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.
2. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Pygopus 2) monoclonal Antibody, unconjugated (TMAB-11959) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

FCM=1:50-100; ICC/IF=1:50-200

Monoclonal

A synthesized peptide: human Pygopus 2

Human

PYGO2

Pygopus homolog 2

Involved in signal transduction through the Wnt pathway.