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Anti-PSGL-1/CD162 Antibody (9H297)

🥰Excellent
Catalog No. TMAY-02649

Anti-PSGL-1/CD162 Antibody (9H297) is a Rabbit antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (9H297) can be used in IHC-P,FCM.

Anti-PSGL-1/CD162 Antibody (9H297)

Anti-PSGL-1/CD162 Antibody (9H297)

🥰Excellent
Catalog No. TMAY-02649
Anti-PSGL-1/CD162 Antibody (9H297) is a Rabbit antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (9H297) can be used in IHC-P,FCM.
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Product Introduction

Bioactivity
Description
Anti-PSGL-1/CD162 Antibody (9H297) is a Rabbit antibody targeting PSGL-1/CD162. Anti-PSGL-1/CD162 Antibody (9H297) can be used in IHC-P,FCM.
Ig Type
Monoclonal Rabbit IgG
Clone
9H297
Reactivity
Human
Verified Activity
1. Immunochemical staining of human SELPLG in human tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

2. Immunochemical staining of human SELPLG in human spleen with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

3. Flow cytometric analysis of Human SELPLG(CD162) expression on human whole blood granulocytes. Cells were stained with purified anti-Human SELPLG(CD162), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes.
Application
IHC-P,FCM
Recommended Dose
IHC-P: 1:100-1:500; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human PSGL-1 / CD162 / SELPLG (rh PSGL-1 / CD162 / SELPLG; TMPY-03531; Q14242-1; Met1-Gly295).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundP-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
APC/FITC/PE
Antibody Types Available
4
Antigen Details
Immunogen
Recombinant Human PSGL-1 / CD162 / SELPLG Protein (TMPY-03531)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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