Anti-presenilin 1 Polyclonal Antibody is a Rabbit antibody targeting presenilin 1. Anti-presenilin 1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Rabbit)

1. Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Spinal cord Lysates
Lane 3: Mouse Testis Lysates
Lane 4: Rat Spinal cord Lysates
Lane 5: Rat Testis Lysates
Lane 6: Human U251 cell Lysates
Lane 7: Human SH-SY5Y cell Lysates
Lane 8: Human MCF-7 cell Lysates
Lane 9: Human THP-1 cell Lysates
Lane 10: Human U-87 MG cell Lysates
Primary: Anti-presenilin 1 (TMAB-11729) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34/52 kDa
Observed band size: 50 kDa
2. Sample:
K562 Cell (Human) Lysate at 40 μg
HepG2 Cell (Human) Lysate at 40 μg
Primary: Anti-presenilin 1 (TMAB-11729) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34/52 kD
Observed band size: 50 kD
3. Sample: K562 Cell Lysate at 40 μg
Primary: Anti-presenilin1 (TMAB-11729) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34/52 kD
Observed band size: 48 kD
4. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (presenilin 1) Polyclonal Antibody, Unconjugated (TMAB-11729) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
5. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (presenilin 1) polyclonal Antibody, Unconjugated (TMAB-11729) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
6. Blank control: Raji.
Primary Antibody (green line): Rabbit Anti-presenilin 1 antibody (TMAB-11729)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Presenilin-1 NTF subunit

Human

PSEN1

Presenilin-1

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.