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Anti-PKM2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01544

Anti-PKM2 Polyclonal Antibody is a Mouse antibody targeting PKM2. Anti-PKM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

Anti-PKM2 Polyclonal Antibody

Anti-PKM2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01544
Anti-PKM2 Polyclonal Antibody is a Mouse antibody targeting PKM2. Anti-PKM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$374 7-10 days
200 μL$527 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PKM2 Polyclonal Antibody is a Mouse antibody targeting PKM2. Anti-PKM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)
Verified Activity
1. Sample:
MOLT-4 (Human) Cell Lysate at 30 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
2. Sample:
Hela Cell (Human) Lysate at 40 μg
NIH/3T3 Cell (Mouse) Lysate at 40 μg
Muscle (Mouse) Lysate at 40 μg
Jurkat Cell (Human) Lysate at 40 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
3. Tissue/cell: rat colitis tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (TMAB-01544) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Mouse IgG, Cy5 conjugated at 37°C for 20 min;
Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (TMAB-01544) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: U87MG (Human) Cell Lysate at 30 μg
Lane 3: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
6. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
7. U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human PKM2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Energy Metabolism,Energy Metabolism,Carbohydrate metabolism,Kinases/Phosphatases,Enzymes,Metabolism of carbohydrates
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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