Shopping Cart
- Remove All
- Your shopping cart is currently empty
Anti-PKM2 Polyclonal Antibody is a Mouse antibody targeting PKM2. Anti-PKM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-PKM2 Polyclonal Antibody is a Mouse antibody targeting PKM2. Anti-PKM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit) |
Verified Activity | 1. Sample: MOLT-4 (Human) Cell Lysate at 30 μg Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa 2. Sample: Hela Cell (Human) Lysate at 40 μg NIH/3T3 Cell (Mouse) Lysate at 40 μg Muscle (Mouse) Lysate at 40 μg Jurkat Cell (Human) Lysate at 40 μg Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa 3. Tissue/cell: rat colitis tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (TMAB-01544) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Mouse IgG, Cy5 conjugated at 37°C for 20 min; Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (TMAB-01544) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 5. Sample: Lane 1: A549 (Human) Cell Lysate at 30 μg Lane 2: U87MG (Human) Cell Lysate at 30 μg Lane 3: U251 (Human) Cell Lysate at 30 μg Primary: Anti-PKM2 (TMAB-01544) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa 6. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 7. U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Mouse |
Subcellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. |
Immunogen | KLH conjugated synthetic peptide: human PKM2 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Energy Metabolism,Energy Metabolism,Carbohydrate metabolism,Kinases/Phosphatases,Enzymes,Metabolism of carbohydrates |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.