Anti-Phospho-STAT5b (Ser731) Polyclonal Antibody 2 is a Rabbit antibody targeting Phospho-STAT5b (Ser731). Anti-Phospho-STAT5b (Ser731) Polyclonal Antibody 2 can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep)

Blank control (Black line): Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-phospho-STAT5b (Ser731) antibody (TMAB-11326)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human STAT5b around the phosphorylation site of Ser731

Human

STAT5B

Signal transducer and activator of transcription 5B

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.