Anti-Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Antibody (1J605) is a Rabbit antibody targeting Phospho-PAK1/2/3 (Ser144/Ser141/Ser154). Anti-Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Antibody (1J605) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,IP,FCM.

IgG

1J605

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated (TMAB-11103) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated (TMAB-11103) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated (TMAB-11103) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) Monoclonal Antibody, Unconjugated (TMAB-11103) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. MCF-7 (H) cells were treated with or without EGF (1 µg/ml) for 10 min, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PAK1/2/3 (Ser144/Ser141/Ser154) monoclonal antibody, unconjugated (TMAB-11103) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

WB: 1:500-5000; IHC-P: 1:100-200; IHC-Fr: 1:100-200; ICC/IF: 1:50-200; IF: 1:100-200; IP: 1:20-50; FCM: 1:50-100

Monoclonal

A synthesized peptide: human PAK1 around the phosphorylation site of S144

Human

PAK1

Serine/threonine-protein kinase PAK 1

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2.