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Anti-Phospho-NFE2L2 (S40) Antibody (7L44) is a Rabbit antibody targeting Phospho-NFE2L2 (S40). Anti-Phospho-NFE2L2 (S40) Antibody (7L44) can be used in WB,IHC,ICC/IF,IP,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $296 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-Phospho-NFE2L2 (Ser40) Antibody (7L44) is a Rabbit antibody targeting Phospho-NFE2L2 (Ser40). Anti-Phospho-NFE2L2 (Ser40) Antibody (7L44) can be used in WB,IHC,ICC/IF,IP,FCM. |
Synonyms | p-NFE2L2 (Ser40), p-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40) |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. Western blot analysis of Phospho-Nrf2 (S40) on different lysates using anti-Phospho-Nrf2 (S40) antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Raji. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-Nrf2 (S40) antibody. Counter stained with hematoxylin. 5. ICC staining Phospho-Nrf2 (S40) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Phospho-Nrf2 (S40) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Phospho-Nrf2 (S40) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of K562 cells with Phospho-Nrf2 (S40) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; IP: 1:50; FCM: 1:50 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. |
Conjucates | Unconjugated |
Others Formats | Phospho |
Immunogen | A synthesized phosphopeptide: human Nrf2 around the phosphorylation site of S40 |
Antigen Species | human |
Uniprot ID |
Molecular Weight | Theoretical: 90 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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