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Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody
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Catalog No. TMAB-10700
Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FGFR1 (Tyr653+Tyr654). Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody
Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-10700
Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FGFR1 (Tyr653+Tyr654). Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody is a Rabbit antibody targeting Phospho-FGFR1 (Tyr653+Tyr654). Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-FGFR1 (Tyr653+Tyr654)) Polyclonal Antibody, Unconjugated (TMAB-10700) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Phospho-FGFR1 (Tyr653+Tyr654) Polyclonal Antibody, Unconjugated (TMAB-10700) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 3. Sample: MCF-7 (Human) Cell Lysate at 30 μg k562 (human) Cell Lysate at 30 μg Primary: Anti-Phospho-FGFR1 (Tyr653+Tyr654) (TMAB-10700) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 128 kD 4. Sample: Embryo (Mouse) Lysate at 40 μg Primary: Anti-Phospho-FGFR1 (Tyr653+Tyr654) (TMAB-10700) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 128 kD 5. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human A673 cell lysates Lane 4: Human Molt-4 cell lysates Primary: Anti-Phospho-FGFR1 (Tyr653+Tyr654) (TMAB-10700) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kDa Observed band size: 135 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle. |
| Tissue Specificity | Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through the cellular surface tyrosine kinase receptors. There are four members of the FGF receptor family: FGFR-1 (flg), FGFR-2 (bek, KGFR), FGFR-3 and FGFR-4. Each receptor contains an extracellular ligand binding domain, a transmembrane region and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR-1 can be phosphorylated: Tyr463, Tyr583, Tyr585, Tyr653, Tyr654, Tyr730 and Tyr766. Tyrosine 653 and 654 are important for catalytic activity of the activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCgamma. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human FGFR1 around the phosphorylation site of Tyr653/654 |
| Antigen Species | Human |
| Gene Name | FGFR1 |
| Gene ID | |
| Protein Name | Fibroblast growth factor receptor 1 |
| Uniprot ID | |
| Function | Receptor for fibroblast growth factors FGF2 and FGF1. Receptor for FGF23 in the presence of KL (By similarity). Promotes mitogenesis in response to fibroblast growth factors. Activates PLCG1. |
| Molecular Weight | Theoretical: 88 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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