Anti-Phospho-Desmin (Thr76+Thr77) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Desmin (Thr76+Thr77). Anti-Phospho-Desmin (Thr76+Thr77) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human

1. Blank control (black line): A431 (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature)
Primary Antibody (green line): Rabbit Anti-Phospho-Desmin (Thr76+Thr77) antibody (TMAB-10596);
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;Dilution: 1 μg /test.
2. Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-Desmin (Thr76+Thr77)) Polyclonal Antibody, Unconjugated (TMAB-10596) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-Desmin (Thr76+Thr77)) Polyclonal Antibody, Unconjugated (TMAB-10596) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human DES around the phosphorylation site of Thr76/Thr77

Human

DES

Desmin

Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures.