Anti-Phospho-Connexin 43 (Ser368) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Connexin 43 (Ser368). Anti-Phospho-Connexin 43 (Ser368) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Mouse (predicted:Human,Rat,Dog,Cow,Monkey)

1. Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-Connexin 43) Polyclonal Antibody, Unconjugated (TMAB-10532) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Blank control: Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37℃). Primary Antibody: Rabbit Anti-Phospho-Connexin 43 (Ser368) antibody (TMAB-10532, Green); Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Connexin 43 around the phosphorylation site of Ser368

Human

GJA1

Gap junction alpha-1 protein

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract.