Anti-Phospho-c-Fos (Ser374) Polyclonal Antibody is a Rabbit antibody targeting Phospho-c-Fos (Ser374). Anti-Phospho-c-Fos (Ser374) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat

1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-c-Fos (Ser374)) Polyclonal Antibody, Unconjugated (TMAB-10492) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-c-Fos (Ser374)) Polyclonal Antibody, Unconjugated (TMAB-10492) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Cerebrum (Rat) Lysate at 40 μg
Primary: Anti-Phospho-c-Fos (Ser374) (TMAB-10492) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62/46 kD
Observed band size: 46 kD
4. Sample: Jurkat Cell Lysate at 30 μg
Primary: Anti-Phospho-c-Fos (Ser374) (TMAB-10492) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 36 kD
5. Sample: NIH/3T3 Cell (Mouse) Lysate at 30 μg
Primary: Anti-Phospho-c-Fos (Ser374) (TMAB-10492) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 36 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human c-Fos around the phosphorylation site of Ser374

Human

FOS

Proto-oncogene c-Fos

Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.