Anti-Phospho-Alpha synuclein (Ser129) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Alpha synuclein (Ser129). Anti-Phospho-Alpha synuclein (Ser129) Polyclonal Antibody can be used in WB,FCM.

IgG

Human

1. Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-phospho-Alpha synucleusn (Ser129) antibody (TMAB-01385)
Dilution: 3 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. 25 μg total protein per Lane of various lysates probed with phospho-Alpha synucleusn polyclonal antibody, unconjugated (TMAB-01385) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human SNCA around the phosphorylation site of Ser129

Human

Tangles & Tau,Synuclein