Anti-Phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody is a Rabbit antibody targeting Phospho-14-3-3 protein zeta (Ser58). Anti-Phospho-14-3-3 protein zeta (Ser58) Polyclonal Antibody can be used in WB,FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit)

1. Sample: HepG2 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-14-3-3 protein zeta (Ser58) (TMAB-10279) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
2. Blank control (blue line): A549 (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 0.1% P Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg /test.

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human 14-3-3 protein zeta/delta around the phosphorylation site of Ser58

Human

YWHAZ

14-3-3 protein zeta/delta

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.