Anti-Peroxiredoxin 1 Antibody (6N118) is a Mouse antibody targeting Peroxiredoxin 1. Anti-Peroxiredoxin 1 Antibody (6N118) can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

6N118

Human,Mouse,Rat

1. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Mouse Cerebrum tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Human Jurkat cell lysates
Lane 7: Human Raji cell lysates
Lane 8: Human MCF-7 cell lysates
Lane 9: Human A431 cell lysates
Lane 10: Human Hela cell lysates
Primary: Anti-Peroxiredoxin 1 (TMAB-10195) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 23 kD
2. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Peroxiredoxin 1) Monoclonal Antibody, Unconjugated (ascites of TMAB-10195 10F9) at 1: 2000 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
3. Blank control (black line): Hela.
Primary Antibody (green line): Mouse Anti-Peroxiredoxin 1 antibody (TMAB-10195)
Dilution:0.5 μg/Test;
Secondary Antibody (white blue line): Goat anti-mouse IgG-FITC
Dilution: 0.5 μg/Test.
Isotype control (orange line): Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.5μg/Test

Monoclonal

Recombinant Protein: human Peroxiredoxin 1 Protein

Human

PRDX1

Peroxiredoxin-1

Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.