Anti-Nitro-Alpha-synuclein (Tyr39) Polyclonal Antibody is a Rabbit antibody targeting Nitro-Alpha-synuclein (Tyr39). Anti-Nitro-Alpha-synuclein (Tyr39) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig)

1. Blank control (Black line): HUVEC (Black).
Primary Antibody (green line): Rabbit Anti-Alpha-synucleusn (nitro-Tyr39) antibody (TMAB-01239)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Alpha-synucleusn (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (TMAB-01239) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Alpha-synucleusn (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (TMAB-01239) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Alpha-synucleusn (nitro-Tyr39)) Polyclonal Antibody, Unconjugated (TMAB-01239) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.

IHC-P,IHC-Fr,IF,FCM

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test

Polyclonal

KLH conjugated Synthesised nitrylpeptide: human SNCA around the nitrosation site of Tyr39

Human

Tangles & Tau,Synuclein