Anti-NFIB Polyclonal Antibody is a Rabbit antibody targeting NFIB. Anti-NFIB Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat,Chicken,Pig,Cow,Rabbit,Sheep)

1. Blank control: A431.
Primary Antibody (green line): Rabbit Anti-NFIB antibody (TMAB-09431)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: A431 (Human) Cell Lysate at 30 μg
Lane 3: Lung (Mouse) Lysate at 40 μg
Lane 4: Ovary (Mouse) Lysate at 40 μg
Lane 5: Kidney (Mouse) Lysate at 40 μg
Primary: Anti-NFIB (TMAB-09431) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
3. Sample: 293T Cell (Human) Lysate at 40 μg
Primary: Anti-NFIB (TMAB-09431) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 47 kD
4. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-NFIB (TMAB-09431) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human NFIB/NF1B2

Human

NFIB

Nuclear factor 1 B-type

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.