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Anti-NFAT2 Antibody (1J539) is a Rabbit antibody targeting NFAT2. Anti-NFAT2 Antibody (1J539) can be used in WB,ICC,IHC,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $298 | 7-10 days | |
100 μL | $497 | 7-10 days |
Description | Anti-NFAT2 Antibody (1J539) is a Rabbit antibody targeting NFAT2. Anti-NFAT2 Antibody (1J539) can be used in WB,ICC,IHC,FCM. |
Synonyms | Nuclear factor of activated T cells cytosolic component 1, Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1, Nuclear factor of activated T cells cytoplasmic 1, NFATc1, NFATC 1, NFATc, NFAT2, NFAT transcription complex cytosolic component, NFAT 2, NFAC1, NF ATc1, NF ATc, MGC138448 |
Ig Type | IgG |
Reactivity | Human |
Verified Activity | 1. Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-NFAT2 antibody. Counter stained with hematoxylin. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human skeleton muscle tissue using anti-NFAT2 antibody. Counter stained with hematoxylin. 5. ICC staining NFAT2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining NFAT2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining NFAT2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Jurkat cells with NFAT2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Application | |
Recommended Dose | IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 101 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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