Anti-Neurturin Polyclonal Antibody is a Rabbit antibody targeting Neurturin. Anti-Neurturin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Cow)

1. Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Neurturin Polyclonal Antibody, Unconjugated (TMAB-09417) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
placenta (Mouse) Lysate at 30 μg;
Brain (Mouse) lysate at 30 μg;
Primary: Anti-Neurturin (TMAB-09417) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000 dilution;
Predicted band size: 21 kD
Observed band size: 21 kD
3. Sample:
Lane 1: Human K562 cell Lysates
Lane 2: Human 293T cell Lysates
Primary: Anti-Neurturin (TMAB-09417) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Neurturin

Human

NRTN

Neurturin

Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells.