Anti-Neurokinin A Polyclonal Antibody is a Rabbit antibody targeting Neurokinin A. Anti-Neurokinin A Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Chicken,Dog,Cow,Horse,Rabbit,Sheep)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated (TMAB-09395) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-NKA/Neurokinin A Polyclonal Antibody, Unconjugated (TMAB-09395) 1: 200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1: 200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml, blue) was used to stain the cell nuclei
3. Sample:
Lane 1: Mouse Spleen tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Human U-2 OS cell lysates
Lane 5: Human SH-SY5Y cell lysates
Lane 6: Human u251 cell lysates
Primary: Anti-Neurokinin A (TMAB-09395) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 11/13 kDa
Observed band size: 11 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human neurokinin A/neuropeptide K

Human

TAC1

Protachykinin-1

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.