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Anti-Nesprin 1 Antibody (1Z207)

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Catalog No. TMAC-02773
Alias SYNE1B, SYNE1_HUMAN, Syne-1, SYNE1, Synaptic nuclei expressed gene 1, Synaptic nuclear envelope protein 1, Spectrin repeat containing nuclear envelope 1, SCAR8, Nuclear envelope spectrin repeat protein 1, Nesprin1, Nesp1, Myocyte nuclear envelope protein 1, Myne-1, MYNE1, Enaptin, EDMD4, dJ45H2.2, CPG2 full length, CPG2, C6orf98, ARCA1, 8B

Anti-Nesprin 1 Antibody (1Z207) is a Rabbit antibody targeting Nesprin 1. Anti-Nesprin 1 Antibody (1Z207) can be used in FCM,ICC,IF,IHC,WB.

Anti-Nesprin 1 Antibody (1Z207)

Anti-Nesprin 1 Antibody (1Z207)

😃Good
Catalog No. TMAC-02773Alias SYNE1B, SYNE1_HUMAN, Syne-1, SYNE1, Synaptic nuclei expressed gene 1, Synaptic nuclear envelope protein 1, Spectrin repeat containing nuclear envelope 1, SCAR8, Nuclear envelope spectrin repeat protein 1, Nesprin1, Nesp1, Myocyte nuclear envelope protein 1, Myne-1, MYNE1, Enaptin, EDMD4, dJ45H2.2, CPG2 full length, CPG2, C6orf98, ARCA1, 8B
Anti-Nesprin 1 Antibody (1Z207) is a Rabbit antibody targeting Nesprin 1. Anti-Nesprin 1 Antibody (1Z207) can be used in FCM,ICC,IF,IHC,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2987-10 days
100 μL$4987-10 days
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Product Introduction

Bioactivity
Description
Anti-Nesprin 1 Antibody (1Z207) is a Rabbit antibody targeting Nesprin 1. Anti-Nesprin 1 Antibody (1Z207) can be used in FCM,ICC,IF,IHC,WB.
SynonymsSYNE1B, SYNE1_HUMAN, Syne-1, SYNE1, Synaptic nuclei expressed gene 1, Synaptic nuclear envelope protein 1, Spectrin repeat containing nuclear envelope 1, SCAR8, Nuclear envelope spectrin repeat protein 1, Nesprin1, Nesp1, Myocyte nuclear envelope protein 1, Myne-1, MYNE1, Enaptin, EDMD4, dJ45H2.2, CPG2 full length, CPG2, C6orf98, ARCA1, 8B
Ig Type
IgG
Clone
1Z207
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution. Positive control: Lane 1: A549, Lane 2: Mouse spleen tissue.
2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
6. Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-Nesprin 1 antibody. Counter stained with hematoxylin.
7. ICC staining Nesprin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining Nesprin 1 in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. ICC staining Nesprin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
10. Flow cytometric analysis of Daudi cells with Nesprin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:500-2000; IHC: 1:50-200; ICC: 1:200-1000; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundMulti-isomeric modular protein which forms a linking network between organelles and the actin cytoskeleton to maintain the subcellular spatial organization. As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. May be involved in nucleus-centrosome attachment and nuclear migration in neural progenitors implicating LINC complex association with SUN1/2 and probably association with cytoplasmic dynein-dynactin motor complexes; SYNE1 and SYNE2 may act redundantly. Required for centrosome migration to the apical cell surface during early ciliogenesis. May be involved in nuclear remodeling during sperm head formation in spermatogenenis; a probable SUN3:SYNE1/KASH1 LINC complex may tether spermatid nuclei to posterior cytoskeletal structures such as the manchette.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthesized phosphopeptide: within Human Nesprin1 aa 8241-8290/8797
Antigen Species
human
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 1011 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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