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Anti-MRF/C11orf9 Polyclonal Antibody
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Catalog No. TMAB-08989
Anti-MRF/C11orf9 Polyclonal Antibody is a Rabbit antibody targeting MRF/C11orf9. Anti-MRF/C11orf9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-MRF/C11orf9 Polyclonal Antibody
Anti-MRF/C11orf9 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08989
Anti-MRF/C11orf9 Polyclonal Antibody is a Rabbit antibody targeting MRF/C11orf9. Anti-MRF/C11orf9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-MRF/C11orf9 Polyclonal Antibody is a Rabbit antibody targeting MRF/C11orf9. Anti-MRF/C11orf9 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated (TMAB-08989) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining 2. Sample: Lung (Mouse) Lysate at 40 μg Primary: Anti-MRF/C11orf9 (TMAB-08989) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD 3. Sample: Lane 1: Human A549 cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human U2OS cell lysates Lane 3: Human A431 cell lysates Primary: Anti-MRF/C11orf9 (TMAB-08989) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa 4. Sample: Uterus (Mouse) Lysate at 40 μg Primary: Anti-MRF/C11orf9 (TMAB-08989) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Membrane. Nucleus. |
| Tissue Specificity | Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | C11orf9 (chromosome 11 open reading frame 9), also known as MRF (myelin gene regulatory factor), Ndt80, KIAA0954 or MGC10781, is a 1,151 amino acid single-pass membrane protein that localizes to nucleus. A member of the MRF family, C11orf9 is expressed in the ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. C11orf9 is weakly expressed in cerebellum and retina. C11orf9 contains one NDT80 DNA-binding domain and acts as a transcription factor that is required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. C11orf9 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 11, which consists of approximately 135 million base pairs and 1,400 genes. Chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. |
| Immunogen | KLH conjugated synthetic peptide: human MRF |
| Antigen Species | Human |
| Gene Name | MYRF |
| Gene ID | |
| Protein Name | Myelin regulatory factor |
| Function | Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes. |
| Molecular Weight | Theoretical: 124 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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