Anti-mouse CD86 Antibody (1E806) is a Rat antibody targeting mouse CD86. Anti-mouse CD86 Antibody (1E806) can be used in FCM,WB.

Rat IgG2a, k

1E806

Mouse

1. scatter diagram showing mouse spleen stained with CD86. The cells were incubated with the antibody (TMAB-08955, 1 µg/Test) for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.Acquisition of >20,000 events was performed.
2. 25 μg total protein per lane of various lysates (see on figure) probed with mouse CD86 monoclonal antibody, unconjugated (TMAB-08955) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.

FCM: 1μg/Test; WB: 1:500-2000

Monoclonal

CD86

T-lymphocyte activation antigen CD86

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.