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Anti-MMP-14 Antibody (7I45)
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Catalog No. TMAC-02619
Alias MT-MMP1, MT1MMP), MT1-MMP, MT 1-MMP, MMP-X1, MMPX1, MMP14, Membrane-type matrix metalloproteinase 1 (MT-MMP 1
Anti-MMP-14 Antibody (7I45) is a Rabbit antibody targeting MMP-14. Anti-MMP-14 Antibody (7I45) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-MMP-14 Antibody (7I45)
Anti-MMP-14 Antibody (7I45)
😃Good
Catalog No. TMAC-02619Alias MT-MMP1, MT1MMP), MT1-MMP, MT 1-MMP, MMP-X1, MMPX1, MMP14, Membrane-type matrix metalloproteinase 1 (MT-MMP 1
Anti-MMP-14 Antibody (7I45) is a Rabbit antibody targeting MMP-14. Anti-MMP-14 Antibody (7I45) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-MMP-14 Antibody (7I45) is a Rabbit antibody targeting MMP-14. Anti-MMP-14 Antibody (7I45) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | MT-MMP1, MT1MMP), MT1-MMP, MT 1-MMP, MMP-X1, MMPX1, MMP14, Membrane-type matrix metalloproteinase 1 (MT-MMP 1 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of MMP14 on human kidney tissue lysates using anti-MMP14 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin. 5. ICC staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MMP14 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:100-200; ICC/IF: 1:50-200; IP 1:20-50; FCM 1:20-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Research Background | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover. |
| Conjucates | Unconjugated |
| Immunogen | A synthesized peptide: human MMP14 |
| Antigen Species | human |
| Uniprot ID |
| Molecular Weight | Theoretical: 65 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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