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Anti-MEK2 Antibody (9Y802)

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Catalog No. TMAC-02541
Alias PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK-1, ERK1

Anti-MEK2 Antibody (9Y802) is a Rabbit antibody targeting MEK2. Anti-MEK2 Antibody (9Y802) can be used in WB,ICC/IF,IHC,IP,FCM.

Anti-MEK2 Antibody (9Y802)

Anti-MEK2 Antibody (9Y802)

😃Good
Catalog No. TMAC-02541Alias PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK-1, ERK1
Anti-MEK2 Antibody (9Y802) is a Rabbit antibody targeting MEK2. Anti-MEK2 Antibody (9Y802) can be used in WB,ICC/IF,IHC,IP,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2967-10 days
100 μL$4977-10 days
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Product Introduction

Bioactivity
Description
Anti-MEK2 Antibody (9Y802) is a Rabbit antibody targeting MEK2. Anti-MEK2 Antibody (9Y802) can be used in WB,ICC/IF,IHC,IP,FCM.
SynonymsPRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK-1, ERK1
Ig Type
IgG
Reactivity
Human,Mouse
Verified Activity
1. Western blot analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Hela, Lane 3: 293T.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
5. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MEK2 antibody. Counter stained with hematoxylin.
6. ICC staining MEK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of Hela cells with MEK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Recommended Dose
WB: 1:1000-5000; IHC: 1:100-500; ICC/IF: 1:100-500; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundA family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 44 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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