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Anti-MEK2 Antibody (9Y802)
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Catalog No. TMAC-02541
Alias PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK-1, ERK1
Anti-MEK2 Antibody (9Y802) is a Rabbit antibody targeting MEK2. Anti-MEK2 Antibody (9Y802) can be used in FCM,ICC/IF,IHC,IP,WB.
Anti-MEK2 Antibody (9Y802)
Anti-MEK2 Antibody (9Y802)
😃Good
Catalog No. TMAC-02541Alias PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK-1, ERK1
Anti-MEK2 Antibody (9Y802) is a Rabbit antibody targeting MEK2. Anti-MEK2 Antibody (9Y802) can be used in FCM,ICC/IF,IHC,IP,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-MEK2 Antibody (9Y802) is a Rabbit antibody targeting MEK2. Anti-MEK2 Antibody (9Y802) can be used in FCM,ICC/IF,IHC,IP,WB. |
| Synonyms | PRKM3, p44-MAPK, P44MAPK, p44-ERK1, P44ERK1, HUMKER1A, HS44KDAP, ERT2, ERK-2, Erk2, ERK-1, ERK1 |
| Ig Type | IgG |
| Clone | 9Y802 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Hela, Lane 3: 293T. 2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MEK2 antibody. Counter stained with hematoxylin. 6. ICC staining MEK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with MEK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.          |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:100-500; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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