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Anti-MEK-7 Antibody (9U312) is a Rabbit antibody targeting MEK-7. Anti-MEK-7 Antibody (9U312) can be used in WB,ICC/IF,IHC,IP,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $496 | 7-10 days |
Description | Anti-MEK-7 Antibody (9U312) is a Rabbit antibody targeting MEK-7. Anti-MEK-7 Antibody (9U312) can be used in WB,ICC/IF,IHC,IP,FCM. |
Synonyms | stress-activated protein kinase kinase 4, SKK4, Sek-2, Sek2, Sek 2, SAPKK-4, SAPKK4, SAPK kinase 4, PRKMK-7, PRKMK7, PRKMK 7, MP2K7_HUMAN, MKK-7, MKK7, MKK 7, Mitogen Activated Protein Kinase kinase 7, MEK7, MAPKK-7, MAPKK7, MAPKK 7, MAPK/ERK kinase 7, MAP2K7, MAP kinase kinase 7, Jnkk-2, Jnkk2, JNKK 2, JNK-activating kinase 2, JNK kinase 2, JNK activating kinase 2, Dual specificity mitogen-activated protein kinase kinase 7, Dual specificity mitogen activated protein kinase kinase 7, c-Jun N-terminal kinase kinase 2 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of MEK7 on different lysates using anti-MEK7 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293T, Lane 3: A431. 2. Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-MEK7 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MEK7 antibody. Counter stained with hematoxylin. 5. ICC staining MEK7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining MEK7 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining MEK7 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with MEK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Application | |
Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 47 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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