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Anti-MAPK1 Antibody (5N256)
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Catalog No. TMAC-01372
Alias Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1
Anti-MAPK1 Antibody (5N256) is a Rabbit antibody targeting MAPK1. Anti-MAPK1 Antibody (5N256) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-MAPK1 Antibody (5N256)
Anti-MAPK1 Antibody (5N256)
😃Good
Catalog No. TMAC-01372Alias Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1
Anti-MAPK1 Antibody (5N256) is a Rabbit antibody targeting MAPK1. Anti-MAPK1 Antibody (5N256) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-MAPK1 Antibody (5N256) is a Rabbit antibody targeting MAPK1. Anti-MAPK1 Antibody (5N256) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | Protein tyrosine kinase ERK 2, Protein kinase mitogen activated 3, Protein kinase mitogen activated 2, Protein kinase mitogen activated 1, PRKM3, PRKM2, PRKM1, PRKM 3, PRKM 2, PRKM 1, p44MAPK, p44ERK1, p44 MAPK, p44 ERK1, p42-MAPK, p42MAPK, p42 MAPK, p41mapk, p41, p40, p38, MK03, MK01, Mitogen activated protein kinase 3, Mitogen activated protein kinase 2, Mitogen activated protein kinase 1, Microtubule associated protein 2 kinase, MGC20180, MAPK3, MAPK2, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 2, MAP kinase 1, Insulin stimulated MAP2 kinase, HUMKER1A, HS44KDAP, Extracellular signal-regulated kinase 2, Extracellular signal regulated kinase 2, Extracellular signal regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK1, ERK 2, ERK 1/2, ERK 1 |
| Ig Type | IgG |
| Clone | 5N256 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: PC-12. 2. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ERK2 antibody. Counter stained with hematoxylin. 5. ICC staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein-coupled receptor ligands, and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK1 and ERK2 requires phosphorylation by upstream kinases such as MAP kinase kinase (MEK), MEK kinase and Raf-1. ERK1 and ERK2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the Threonine-Glutamate-Tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK2 gene maps to chromosome 22q11.21 and encodes a 360-amino acid protein. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 41 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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