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Anti-Lipin 1 Antibody (5B766)
😃Good
Catalog No. TMAC-02404
Alias Phosphatidate phosphatase LPIN1, PAP1, LPIN1_HUMAN, Lpin1, Lipin-1, KIAA0188, EC=3.1.3.4
Anti-Lipin 1 Antibody (5B766) is a Rabbit antibody targeting Lipin 1. Anti-Lipin 1 Antibody (5B766) can be used in WB,ICC,IF,IHC,FCM,IP.
Anti-Lipin 1 Antibody (5B766)
Anti-Lipin 1 Antibody (5B766)
😃Good
Catalog No. TMAC-02404Alias Phosphatidate phosphatase LPIN1, PAP1, LPIN1_HUMAN, Lpin1, Lipin-1, KIAA0188, EC=3.1.3.4
Anti-Lipin 1 Antibody (5B766) is a Rabbit antibody targeting Lipin 1. Anti-Lipin 1 Antibody (5B766) can be used in WB,ICC,IF,IHC,FCM,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Chemical Properties
| Description | Anti-Lipin 1 Antibody (5B766) is a Rabbit antibody targeting Lipin 1. Anti-Lipin 1 Antibody (5B766) can be used in WB,ICC,IF,IHC,FCM,IP. |
| Synonyms | Phosphatidate phosphatase LPIN1, PAP1, LPIN1_HUMAN, Lpin1, Lipin-1, KIAA0188, EC=3.1.3.4 |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Lipin 1 on different cell lysates using anti-Lipin 1 at 1/500 dilution. Positive control: Line 1: K562, Line 2: SH-SY-5Y, Line 3: Mouse lung. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin. 5. ICC staining Lipin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Lipin 1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of PC-3M cells with Lipin 1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:500-1000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity). |
| Conjucates | Unconjugated |
| Uniprot ID |
| Molecular Weight | Theoretical: 130 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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