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Anti-KRT7 Polyclonal Antibody is a Rabbit antibody targeting KRT7. Anti-KRT7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $374 | 7-10 days | |
200 μL | $527 | 7-10 days |
Description | Anti-KRT7 Polyclonal Antibody is a Rabbit antibody targeting KRT7. Anti-KRT7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (TMAB-01041) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK7) Polyclonal Antibody, Unconjugated (TMAB-01041) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Bronchus (Mouse) Lysate at 40 μg Primary: Anti-CK7 (TMAB-01041) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 4. Sample: Skin (Mouse) Lysate at 40 μg Primary: Anti-CK7 (TMAB-01041) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 5. Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CK7 Polyclonal Antibody, Unconjugated (TMAB-01041) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 6. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CK7 Polyclonal Antibody, Unconjugated (TMAB-01041) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 7. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CK7 Polyclonal Antibody, Unconjugated (TMAB-01041) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 8. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CK7 Polyclonal Antibody, Unconjugated (TMAB-01041) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 9. Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CK7 Polyclonal Antibody, Unconjugated (TMAB-01041) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 10. Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-CK7 Polyclonal Antibody, Unconjugated (TMAB-01041) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 11. Blank control: Hepg2 (blue) Isotype Control Antibody: Rabbit IgG-FITC (orange); Primary Antibody Dilution: 1 μL in 100 μL 1X PBS containing 0.5% BSA (green). 12. Blank control: A549. Primary Antibody (green line): Rabbit Anti-CK7 antibody (TMAB-01041) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE Dilution: 0.2 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 13. Blank control: Hela. Primary Antibody (green line): Rabbit Anti-CK7 antibody (TMAB-01041) Dilution: 1 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 14. Sample: Lane 1: Mouse Urinary bladder tissue lysates Lane 2: Mouse Breast tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Mouse trachea tissue lysates Lane 5: Rat Urinary bladder tissue lysates Lane 6: Rat Placenta tissue lysates Lane 7: Human Hela cell lysates Lane 8: Human Hepg2 cell lysates Lane 9: Human Siha cell lysates Lane 10: Human Huvec cell lysates Primary: Anti-CK7 (TMAB-01041) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 0.2µg/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
Immunogen | KLH conjugated synthetic peptide: the middle of human CK7 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Cytokeratins |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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