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Anti-KRT7 Antibody (4X26) (Synonyms: Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, K...)

Catalog No. TMAC-01093 Copy Product Info
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Anti-KRT7 Antibody (4X26) is a Rabbit antibody targeting KRT7. Anti-KRT7 Antibody (4X26) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-KRT7 Antibody (4X26)

Copy Product Info
🥰Excellent
Catalog No. TMAC-01093
Synonyms Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7)

Anti-KRT7 Antibody (4X26) is a Rabbit antibody targeting KRT7. Anti-KRT7 Antibody (4X26) can be used in FCM,ICC/IF,IHC,IP,WB.

Anti-KRT7 Antibody
(4X26)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2987-10 days7-10 days
100 μL$4977-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-KRT7 Antibody (4X26) is a Rabbit antibody targeting KRT7. Anti-KRT7 Antibody (4X26) can be used in FCM,ICC/IF,IHC,IP,WB.
SynonymsType-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7)
Ig Type
IgG
Clone
4X26
Reactivity
Human
Verified Activity
1. Western blot analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: HepG2, Lane 3: SK-BR-3, Lane 4: SW1990.
2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin.
4. ICC staining Cytokeratin 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
5. ICC staining Cytokeratin 7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining Cytokeratin 7 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. Flow cytometric analysis of Hela cells with Cytokeratin 7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Application
Recommended Dose
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionRecombinant Antibody
PurificationProA affinity purified
AppearanceLiquid
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Research BackgroundCytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 51 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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