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Anti-KRT7 Antibody (4X26)
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Catalog No. TMAC-01093
Alias Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7)
Anti-KRT7 Antibody (4X26) is a Rabbit antibody targeting KRT7. Anti-KRT7 Antibody (4X26) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-KRT7 Antibody (4X26)
Anti-KRT7 Antibody (4X26)
😃Good
Catalog No. TMAC-01093Alias Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7)
Anti-KRT7 Antibody (4X26) is a Rabbit antibody targeting KRT7. Anti-KRT7 Antibody (4X26) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-KRT7 Antibody (4X26) is a Rabbit antibody targeting KRT7. Anti-KRT7 Antibody (4X26) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | Type-II keratin Kb7, type II cytoskeletal 7, SCL, Sarcolectin, KRT7, Keratin-7 (K7), Keratin, type II cytoskeletal 7, Cytokeratin-7 (CK-7) |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Cytokeratin 7 on different lysates using anti-Cytokeratin 7 antibody at 1/1,000 dilution. Positive control: Lane 1: A549, Lane 2: HepG2, Lane 3: SK-BR-3, Lane 4: SW1990. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 7 antibody. Counter stained with hematoxylin. 4. ICC staining Cytokeratin 7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining Cytokeratin 7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cytokeratin 7 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of Hela cells with Cytokeratin 7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 51 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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