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Anti-Kinesin 5B Polyclonal Antibody

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Catalog No. TMAB-08066

Anti-Kinesin 5B Polyclonal Antibody is a Rabbit antibody targeting Kinesin 5B. Anti-Kinesin 5B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-Kinesin 5B Polyclonal Antibody

Anti-Kinesin 5B Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-08066
Anti-Kinesin 5B Polyclonal Antibody is a Rabbit antibody targeting Kinesin 5B. Anti-Kinesin 5B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-Kinesin 5B Polyclonal Antibody is a Rabbit antibody targeting Kinesin 5B. Anti-Kinesin 5B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse,Rat (predicted:Human,Dog,Pig,Cow,Horse,Sheep)
Verified Activity
1. Sample: heart (Mouse) Lysate at 40 μg
Primary: Anti-Kinesin 5B at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
2. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (TMAB-08066) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (TMAB-08066) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityExpressed only in testis and placenta.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundThis gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human KLF5
Antigen Species
Human
Gene Name
KLF5
Gene ID
Protein Name
Krueppel-like factor 5
Uniprot ID
Function
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Chemical Properties
Molecular WeightTheoretical: 50 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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