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Anti-KDM3A Antibody (5B591)
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Catalog No. TMAC-02301
Alias TSGA, Testis specific protein A, Lysine-specific demethylase 3A, Lysine (K) specific demethylase 3A, KIAA0742, KDM3A_HUMAN, Kdm 3a, Jumonji domain-containing protein 1A, Jumonji domain containing protein 1A, Jumonji domain containing 1A, Jumonji domain containing 1, Jumonji C domain containing histone demethylase 2A, JMJD1A, JMJD1, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A, DKFZp686P07111, DKFZp686A24246
Anti-KDM3A Antibody (5B591) is a Mouse antibody targeting KDM3A. Anti-KDM3A Antibody (5B591) can be used in WB,ICC,FCM.
Anti-KDM3A Antibody (5B591)
Anti-KDM3A Antibody (5B591)
😃Good
Catalog No. TMAC-02301Alias TSGA, Testis specific protein A, Lysine-specific demethylase 3A, Lysine (K) specific demethylase 3A, KIAA0742, KDM3A_HUMAN, Kdm 3a, Jumonji domain-containing protein 1A, Jumonji domain containing protein 1A, Jumonji domain containing 1A, Jumonji domain containing 1, Jumonji C domain containing histone demethylase 2A, JMJD1A, JMJD1, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A, DKFZp686P07111, DKFZp686A24246
Anti-KDM3A Antibody (5B591) is a Mouse antibody targeting KDM3A. Anti-KDM3A Antibody (5B591) can be used in WB,ICC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $426 | 7-10 days |
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| Description | Anti-KDM3A Antibody (5B591) is a Mouse antibody targeting KDM3A. Anti-KDM3A Antibody (5B591) can be used in WB,ICC,FCM. |
| Synonyms | TSGA, Testis specific protein A, Lysine-specific demethylase 3A, Lysine (K) specific demethylase 3A, KIAA0742, KDM3A_HUMAN, Kdm 3a, Jumonji domain-containing protein 1A, Jumonji domain containing protein 1A, Jumonji domain containing 1A, Jumonji domain containing 1, Jumonji C domain containing histone demethylase 2A, JMJD1A, JMJD1, JmjC domain-containing histone demethylation protein 2A, JHMD2A, JHDM2A, DKFZp686P07111, DKFZp686A24246 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of KDM3A on human KDM3A recombinant protein using anti-KDM3A antibody at 1/1,000 dilution. 2. Western blot analysis of KDM3A on HEK293 (1) and KDM3A-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM3A antibody at 1/1,000 dilution. 3. ICC staining KDM3A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. Flow cytometric analysis of Hela cells with KDM3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC: 1:50-200; FCM: 1:100-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | JMJD1A (Jumonji domain containing 1A), also known as TSGA (testis-specific protein A), JMJD1, KDM3A, JHDM2A (JMJC domain-containing histone demethylation protein 2A) or JHMD2A, is a member of the JHDM2 histone demethylase family of proteins that is predominantly expressed in testis. Containing one JMJC domain and a C-terminal C2HC4 zinc finger, JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and a-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 147 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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