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Anti-KAP1 Antibody (4I621)
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Catalog No. TMAC-02296
Alias Tripartite motif-containing protein 28, tripartite motif containing protein 28, Tripartite motif containing 28, Trim28, Transcription intermediary factor 1-beta, Transcription intermediary factor 1 beta, TIF1-beta, TIF1B_HUMAN, TIF1B, TIF1 beta, TF1B, RNF96, RING finger protein 96, Nuclear corepressor KAP-1, Nuclear corepressor KAP 1, KRIP-1, KRIP1, KRIP 1, KRAB-interacting protein 1, KRAB-associated protein 1, KRAB interacting protein 1, KRAB associated protein 1, KAP 1, FLJ29029, E3 SUMO-protein ligase TRIM28, E3 SUMO protein ligase TRIM28
Anti-KAP1 Antibody (4I621) is a Rabbit antibody targeting KAP1. Anti-KAP1 Antibody (4I621) can be used in WB,ICC/IF,IHC,FCM.
Anti-KAP1 Antibody (4I621)
Anti-KAP1 Antibody (4I621)
😃Good
Catalog No. TMAC-02296Alias Tripartite motif-containing protein 28, tripartite motif containing protein 28, Tripartite motif containing 28, Trim28, Transcription intermediary factor 1-beta, Transcription intermediary factor 1 beta, TIF1-beta, TIF1B_HUMAN, TIF1B, TIF1 beta, TF1B, RNF96, RING finger protein 96, Nuclear corepressor KAP-1, Nuclear corepressor KAP 1, KRIP-1, KRIP1, KRIP 1, KRAB-interacting protein 1, KRAB-associated protein 1, KRAB interacting protein 1, KRAB associated protein 1, KAP 1, FLJ29029, E3 SUMO-protein ligase TRIM28, E3 SUMO protein ligase TRIM28
Anti-KAP1 Antibody (4I621) is a Rabbit antibody targeting KAP1. Anti-KAP1 Antibody (4I621) can be used in WB,ICC/IF,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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| Description | Anti-KAP1 Antibody (4I621) is a Rabbit antibody targeting KAP1. Anti-KAP1 Antibody (4I621) can be used in WB,ICC/IF,IHC,FCM. |
| Synonyms | Tripartite motif-containing protein 28, tripartite motif containing protein 28, Tripartite motif containing 28, Trim28, Transcription intermediary factor 1-beta, Transcription intermediary factor 1 beta, TIF1-beta, TIF1B_HUMAN, TIF1B, TIF1 beta, TF1B, RNF96, RING finger protein 96, Nuclear corepressor KAP-1, Nuclear corepressor KAP 1, KRIP-1, KRIP1, KRIP 1, KRAB-interacting protein 1, KRAB-associated protein 1, KRAB interacting protein 1, KRAB associated protein 1, KAP 1, FLJ29029, E3 SUMO-protein ligase TRIM28, E3 SUMO protein ligase TRIM28 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of KAP1 on different lysates using anti-KAP1 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2, Lane 2: Hela. 2. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KAP1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-KAP1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-KAP1 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KAP1 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KAP1 antibody. Counter stained with hematoxylin. 7. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-KAP1 antibody. Counter stained with hematoxylin. 8. ICC staining KAP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. Flow cytometric analysis of Hela cells with KAP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:2000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | TIF1β, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1β and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. Like TIF1α, TIF1β contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression. TIF1β mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger. The human TIF1β gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 110 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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