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Anti-IL12B Polyclonal Antibody 2

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Catalog No. TMAB-07599

Anti-IL12B Polyclonal Antibody 2 is a Rabbit antibody targeting IL12B. Anti-IL12B Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.

Anti-IL12B Polyclonal Antibody 2

Anti-IL12B Polyclonal Antibody 2

Copy Product Info
😃Good
Catalog No. TMAB-07599
Anti-IL12B Polyclonal Antibody 2 is a Rabbit antibody targeting IL12B. Anti-IL12B Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2237-10 days7-10 days
100 μL$3747-10 days7-10 days
200 μL$5297-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-IL12B Polyclonal Antibody 2 is a Rabbit antibody targeting IL12B. Anti-IL12B Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Pig,Cow,Horse,Sheep)
Verified Activity
1. Protein: spleen (mouse) lysates at 40 μg;
Primary: Anti-Il-12 beta (TMAB-07599) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;
ECL excitated the fluorescence;
Predicted band size: 35 kD
Observed band size: 37 kD
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IL-12 beta Polyclonal Antibody, Unconjugated (TMAB-07599) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human IL12B
Antigen Species
Human
Gene Name
IL-12B
Protein Name
Interleukin-12 subunit beta
Chemical Properties
Molecular WeightTheoretical: 35 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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