Anti-IL-1R1 Polyclonal Antibody 2 is a Rabbit antibody targeting IL-1R1. Anti-IL-1R1 Polyclonal Antibody 2 can be used in WB,FCM.

IgG

Human,Mouse (predicted:Rat)

1. Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-IL-1R1 antibody (TMAB-07624);
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE;
Dilution: 1 μg /test.
Protocol
The cells were fixed with 70% methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
2. Sample: Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-IL-1R1 (TMAB-07624) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human IL-1R1

Human

IL1R1

Interleukin-1 receptor type 1

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex.